Tumour vs. normal allele fraction for the three PASS calls independently matched to COLO829's own documented driver mutations — the pattern that separates a real somatic driver from a passenger call.
All three drivers show the expected somatic signature: high tumour AF against near-zero normal-sample background (sequencing noise, not a real germline signal).
chr7:140,753,336 A>T · c.1799T>A · the canonical BRAF hotspot mutation, heterozygous. Confirmed against OncoKB and GeneBe independently.
chr9:21,971,154 CCG>C · p.Ala68Glyfs*51 · a 2bp deletion, homozygous — the near-1.0 tumour AF matches the documented homozygous state.
chr5:1,295,113 GG>AA · reverse complement of the documented CC>TT promoter change · the classic UV-damage dinucleotide substitution.